A structural model for desmosine cross-linked peptides
نویسندگان
چکیده
منابع مشابه
A structural model for desmosine cross-linked peptides.
Desmosine-enriched peptides were isolated from a thermolysin digest of bovine ligamentum nuchae elastin and a partial sequence was determined. A 'two-cross-link' model is proposed in which a second cross-link, perhaps lysinonorleucine, joins two peptide chains approx. 35 amino acid residues removed from the desmosine. Implied in this model is a certain asymmetry or directionality which places r...
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We designed bioinspired cross-linkers based on desmosine, the cross-linker in natural elastin, to prepare hydrogels with thiolated hyaluronic acid. These short, rigid cross-linkers are based on pyridinium salts (as in desmosine) and can connect two polymer backbones. Generally, the obtained semi-synthetic hydrogels are form-stable, can withstand repeated stress, have a large linear-elastic rang...
متن کاملTitle An Enzyme-Linked Immunosorbent Assay for Desmosine
SUMMARY A sensitive, reproducible and rapid analytical method that is capable of identifying the presence of lung damage at an early stage is required, We have developed a sensitive enzyme-linked immunosorbent assay for desmosine in human body fluids. An amino group of des-mosine was exclusively linked to bovine gamma globulin and the high-titer antibody to this conjugate was prepared. This ant...
متن کاملMembrane permeability of hydrocarbon-cross-linked peptides.
Schafmeister, Po, and Verdine (another study) introduced a method using a hydrocarbon linker (staple) to stabilize a peptide in a helical configuration. One intended goal of this scheme is to facilitate the delivery of peptide drugs into target cells. Here, we investigate whether stapled peptides are intrinsically membrane permeable, by performing a case study on a stapled 12-mer peptide named ...
متن کاملDetecting cross-linked peptides by searching against a database of cross-linked peptide pairs.
Mass spectrometric identification of cross-linked peptides can provide valuable information about the structure of protein complexes. We describe a straightforward database search scheme that identifies and assigns statistical confidence estimates to spectra from cross-linked peptides. The method is well suited to targeted analysis of a single protein complex, without requiring an isotope label...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1730617